吴允昆 教授

发布时间: 2017-09-29      访问次数: 167

吴允昆 博士 教授 博士生导师

国家“千人计划(青年项目)”、中国科学院“百人计划”

福建省引进高层次创业创新人才

研究方向:蛋白质结构与功能;基于结构的分子调控剂研发

电话:0591-22868831; 电子邮件:wuyk@fjnu.edu.cn

地址:福州闽侯区福建师范大学旗山校区理工楼11# 513

学习和工作经历

1992.09 - 1996.06  武汉大学生命科学学院                        学士

2002.12 - 2007.06  美国University of Alabama at Birmingham     博士

2007.07 - 2007.12  美国University of Alabama at Birmingham     博士后 

2008.01 - 2011.06  美国HHMI/UT Southwestern                    博士后/研究员

2011.07 - 2017.06  中国科学院福建物质结构研究所           研究员/课题组长、学位/学术委员会委员

2017.07 -          福建师范大学生命科学学院/南方生物医学中心    教授                         


研究简介

本研究组主要综合运用结构生物学手段解析重要功能蛋白的空间结构,通过高分辨率结构深入阐释蛋白机器的作用机制,进而理解其生物功能或病生机理,成果以第一作者在NatureNature Structural & Molecular Biology等、以通讯作者在Plant Cell等杂志上发表。

在蛋白质结构与功能的基础上,结合计算生物和化学合成等手段,设计开发基于特定蛋白靶的分子调控性药物以及运载系统,以实现靶蛋白的应用及改造,主要致力于新型疫苗、抗体、多肽和小分子药物等及其功能运载体的研发,成果以通讯作者发表在Biomaterials等杂志。


研究生获奖  

赵艳和  2011级博士,2016年荣获中国科学院福建物质结构研究所优秀毕业生一等奖

吴秀玲  2012 级博士, 2013年荣获国家奖学金

  

研究组热忱邀请感兴趣的学生报考硕士、博士研究生!!!

研究组具备充足的科研经费及一流的科研环境和实验设备,诚聘教授/副教授/讲师/研究助理,欢迎有志于科研的青年才俊加盟团队。同时欢迎热爱科研工作的本科生参与科研课题。

  

代表性论文* 通讯作者;# 共同第一作者)

1.Wu Y, Yang Y, Ye S, Jiang Y. (2010) Structure of the Intracellular Gating Ring from the Human High-conductance Ca2+-gated K+ Channel. Nature  466(7304):393-7.

2.Wu Y, Sha B. (2006). Crystal structure of yeast mitochondrial outer membrane translocon member Tom70p. Nature Structural & Molecular Biology  13(7):589-93.

3.Li S, Zhao Y, Zhao Z, Wu X, Sun L, Liu Q, Wu Y*. (2016) Crystal structure of GRAS domain of SCARECROW-LIKE 7 in Oryza sativa. Plant Cell  2016 May;28(5):1025-34 DOI: 10.1105/tpc.16.00018 (highlighted article, 生物1区; IF 9.338)

4.Zhang L, Zeng Z, Hu C, Bellis SL, Yang W, Su Y, Zhang X, Wu Y*. (2016) Controlled and targeted release of antigens by intelligent shell for improving applicability of oral vaccines. Biomaterials 2016 Jan;77:307-19. doi: 10.1016/j.biomaterials.2015.11.009. (生物材料1区;IF:8.557)

5.Zhang L*, Fan Y, Wu Y*. (2014) Inositol based none-viral vectors for transgene expression in human cervical carcinoma and hepatoma cell lines. Biomaterials  2014 Feb;35(6):2039-50. doi: 10.1016/j.biomaterials.2013.11.034.  (生物材料1区;IF:8.557)        

6.A luminescent layered hybrid Ag–Ru/LDH as a photocatalytic antibacterial agent. Fangnan Xiao, Xiaolin Liu, Yue Xiao, Fangman Chen,Yunkun Wu*. New Journal of Chemistry 2017 June 41 7260-7266

7.Sun L, Chen P, Su Y, Cai Z, Ruan L, Xu X, Wu Y*. Crystal structure of thermostable alkylsulfatase SdsAP fromPseudomonas sp. S9.Biosci Rep. 2017 May 11;37(3) doi: 10.1042/BSR20170001. 

8.Crystal structure of the type IV secretion system component CagX from Helicobacter pylori.Zhang J, Fan F, Zhao Y, Sun L, Liu Y, Keegan RM, Isupov MN, Wu Y*.Acta Crystallogr F Struct Biol Commun. 2017 Mar 1;73(Pt 3):167-173. doi:10.1107/S2053230X17001376. Epub 2017 Feb 28.

9.Lifang Sun, Yintao Su, Yanhe Zhao, Zheng-qing Fu, Yunkun Wu*.(2016)Crystal Structure of Major Envelope Protein VP24 from White Spot Syndrome Virus. Scientific Reports.2016 Aug 30;6:32309. doi: 10.1038/srep32309. (IF 5.228)

10.Zhang L, Hu C, Yang W, Liu X, Wu Y*. (2016)Chemical synthesis, versatile structures and functions of tailorable adjuvants for optimizing oral vaccination. ACS Appl Mater Interfaces. 2016 Dec 28;8(51):34933-34950. doi: 10.1021/acsami.6b10470  (工程技术一区;IF: 7.145)

11.Shengping Li, Yintao Su, Yadan Liu, Lifang Sun, Minxiang Yu, Yunkun Wu*. (2016)Preparation and characterization of cross-linked enzyme aggregates (CLEAs) of recombinant thermostable alkylsulfatase (SdsAP) from Pseudomonas sp. S9. Process Biochemistry51(12):2084–2089. DOI:10.1016/j.procbio.2016.09.013

12.Lifang Sun, Yunkun Wu*. (2016) Envelope protein VP24 from White spot syndrome virus: expression, purification and crystallization.Acta Crystallogr F Struct Biol Commun. 2016 Aug;72(Pt 8):586-90. doi: 10.1107/S2053230X16009055.

13.Xianren Ye, Yanhe Zhao, Xiuling Wu, Yintao Su, Yunkun Wu*, Feifei She*. (2015) Identification of residues involved in nucleotidyltransferase activity of JHP933 from helicobacter pyloriby site-directed mutagenesis. Archives of Biological Sciences 01/2015; DOI:10.2298/ABS150622127Y 

14.Su Y, Shi F, Zeng Z, Wu X, Zhao Y, Zhang L, Xie Z*, Wu Y*.(2015) A Versatile Monoclonal Antibody Specific Against Human DAB2IP.Monoclon Antib Immunodiagn Immunother. Aug;34(4):246-50. doi: 10.1089/mab.2015.0012.

15.Zhang L*, Bellis SL, Fan Y, Wu Y*. (2015) Using Inositol as a Biocompatible Ligand for Efficient Transgene Expression.International Journal of Nanomedicine. 2015:10 2871-84. (工程技术一区;IF: 4.32)

16.Zhao Y, Ye X, Su Y, Sun L, She F*, Wu Y*. (2014) Crystal structure confirmation of JHP933 as a nucleotidyltransferase superfamily protein from Helicobacter pylori strain J99. PLoS One 2014 Aug 7;9(8):e104609. doi: 10.1371/journal.pone.0104609.

17.Zhang L*, Hu C, Fan Y, Wu Y*. (2013) Binary gene vectors based on hyperbranched poly(L-lactide-co-polyglycerol) and polyethylenimine for prolonged transgene expression via co-assembly with DNA into fiber core–shell triplexes.  J. Mater. Chem. B1(45), 6271-6282  (工程技术一区;IF: 6.627)

18.Li J#, Wu Y*, Qian X, Sha B. (2006). Crystal structure of yeast Sis1 peptide-binding fragment and Hsp70 Ssa1 C-terminal complex. Biochemical Journal398(3): 353-60.

19.Wu Y, McCombs D, Nagy L, Delucas L, Sha B. (2006). Preliminary X-ray crystallographic studies of yeast mitochondrial protein Tom70p. Acta Crystallogr Sect F Struct Biol Cryst Commun. 62(Pt 3):265-7.

20.Wu Y, Li J, Jin Z, Fu Z, Sha B. (2005). The crystal structure of the C-terminal fragment of yeast Hsp40 Ydj1 reveals novel dimerization motif for Hsp40. Journal of Molecular Biology 346(4):1005-11.

21.Tao J, Wu Y, Ron D, Sha B. (2008). Preliminary X-ray crystallographic studies of mouse UPR responsive protein P58 (IPK) TPR fragment.Acta Crystallogr Sect F Struct Biol Cryst Commun. 64(Pt 2):108-10.

22.Hu J, Wu Y, Li J, Qian X, Fu Z, Sha B. (2008) The crystal structure of the putative peptide-binding fragment from the human Hsp40 protein Hdj1. BMC Structural Biology 8:3.


发明专利

1.一种用于PEI等阳离子聚合物介导转染的聚酯保护壳层的熔融超声自组装制备技术申请号 201310642103.3

2. 一种抗人DAB2IP的单克隆抗体及其细胞株  申请号:201410009739.9

3.一种DAB2IP蛋白的ELISA检测方法 (已授权)  申请号:ZL 201410052911.9

4.一种大肠杆菌分泌表达外源蛋白的质粒载体的构建 (已授权)  申请号:201410070632.5

5.一种pH敏感的双壳层载药缓释体系及其制备方法和用途申请号:CN201510063237.9

6.一种口服纳米疫苗运载体的免疫实施与检测技术申请号:201510729106X

7.种钌配合物联合纳米银复合水滑石型纳米材料及其制备方法和用途申请号:CN201610910246.1

8.一种识别重金属铜离子的荧光探针及其合成和应用申请号:201710408711.6

  

研究组热忱邀请感兴趣的学生报考硕士、博士研究生!!!

研究组具备充足的科研经费及一流的科研环境和实验设备,诚聘教授/副教授/讲师/研究助理,欢迎有志于科研的青年才俊加盟团队。同时欢迎热爱科研工作的本科生参与科研课题。