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黄义德教授

时间：2015-03-04浏览：10209

姓名

 黄义德

 性别

 男

 职称学位

教授 、博士

 联系方式


 e-mail

ydhuang@fjnu.edu.cn

  

  

个人简介：黄义德，教授，硕士生导师。


教育经历：1995年福建师范大学生物教育本科毕业，2001年获理学硕士，专业微生物学与生物化学，2005-2008年在福建农林大学攻读博士学位，专业生物化学与分子生物学。


工作经历：2002至今，福建师大生命科学院讲师、副教授、教授。其间分别到美国北亚利桑那大学（Northern Arizona University）和英国爱丁堡大学（University of Edinburgh）癌症研究中心进修学习。


教学：现承担本科 “分子生物学”和研究生“基因工程”、“分子生物学技术”等课程教学工作。


硕士生招生方向：细胞生物学；生物与医药（专硕）


研究方向：

(1)毕赤酵母（Pichia pastoris）表达系统研究

主要研究工作：毕赤酵母表达外源蛋白时细胞生理化学变化的分子机制、毕赤酵母提高外源蛋白的工程化改造、利用毕赤酵母表达系统生产活性蛋白和小分子化合物。

（2）癌症疾病研究

主要研究工作：癌症疾病发生发展的相关分子机制、抗体药物对癌症疾病的治疗和早期诊断。

（3）新型光动力治疗材料的开发与生物应用

主要研究工作：新型光动力治疗材料的开发、光动力治疗材料在抗肿瘤和抗菌方向的应用。


在研项目：

（1） 横向，优化的Komagataella pastoris酵母α-factor信号肽及应用，2022-2025，主持。

（2）福建省自然科学基金，毕赤酵母调控滞留胞内分泌蛋白机制研究，2022-2025，主持。

（3）横向，人体微量THC快速检测技术研发，2021-2023，主持。

（4）省卫生厅，Mycoplasma amphoriforme毒力因子生物信息分析及重组表达，2021-2023，主持。


主要研究论文:

  1.  Chenwei Zou, Lingfang Lu, Shengyan Wang, Chenshan Zhang, Xuequn Chen, Yao Lin and Yide Huang*. The α-mating factor secretion signals and endogenous signal peptides for recombinant protein secretion in Komagataella phaffii. Biotechnology for Biofuels and Bioproducts. 2022 December 16; 15:140

  2.  Yafei Zhang, Shengyan Wang, Lingfang Lu, Chenshan Zhang, Fan Cai, Yao Lin, Yide Huang*. Isolation and evaluation of strong endogenous promoters for the heterologous expression of proteins in Pichia pastoris. (2022) 38:226. World Journal of Microbiology and Biotechnology. 2022 Sep 19;38(12):226.

  3. Yide Huang *, Ting Lin, Lingfang Lu , Fan Cai, Jie Lin, Yu E Jiang*, Yao Lin*. Codon pair optimization (CPO): a software tool for synthetic gene design based on codon pair bias to improve the expression of recombinant proteins in Pichia pastoris. Microb Cell Fact. 2021 Nov 4;20(1):209.

  4.  Qiuhao Ye, Shuanghuang Xiao, Ting Lin, Yufeng Jiang, Yiru Peng * and Yide Huang*. Mesoporous silica-coated gold nanorods loaded with tetrazolyl phthalocyanine as NIR light-activated nano-switches for synergistic photothermal and photodynamic inactivation of antibiotic-resistant Escherichia coli. Materials Advances.2021, 2, 1695-1705.

  5. Huang Y, Wang C, Li K, Ye Y, Shen A, Guo L, Chen P, Meng C, Wang Q, Yang X,Huang Z, Xing X, Lin Y, Liu X, Peng J, Lin Y. Death-associated protein kinase 1suppresses hepatocellular carcinoma cell migration and invasion by upregulationof DEAD-box helicase 20. Cancer Sci. 2020 Aug;111(8):2803-2813.

  6. Yide Huang*, Yafei Zhang, Suhuan Li, Ting Lin, Jingwen Wu & Yao Lin*. Screening for functional IRESes using α-complementation system of β-galactosidase in Pichia pastoris. Biotechnol Biofuels. 2019 Dec 27;12:300. doi: 10.1186/s13068-019-1640-3.

  7.  Xiuqin Chen, Qiumei Guo, Shiqing Dong, Jianling Chen, Shusen Xie, Dongdong Ma,Limin Chen, Hongqin Yang, Yide Huang,* and Yiru Peng*Distribution, Trafficking, and in Vitro Photodynamic Therapy Efficacy of Cholesterol Silicon(IV) Phthalocyanine and Its Nanoparticles in Breast Cancer Cells. ACS Applied Bio Materials. 2019. DOI: 10.1021/acsabm.9b00909

  8.  Dongdong Ma, Xiuqin Chen, Yuhua Wang, Qiumei Guo, Qiuhao Ye, Ruotao Guo Shuanghuang Xiao, Qing Ye, Yide Huang⁎, Yiru Peng⁎. Benzyl ester dendrimer silicon phthalocyanine based polymeric nanoparticle for in vitro photodynamic therapy of glioma. Journal of Luminescence 207 (2019) 597–601.

  9.  Chen X, Wu S, Ma D, Chen J, Guo Q, Han X, Chen K, Yang H, Huang Y⁎, Peng Y⁎. A polyfluoroalkyl substituted phthalocyanine based supramolecular light switch for photothermal and photodynamic antibacterial activity against Escherichia coli. Chem Commun (Camb). 2018 Nov 22;54(94):13279-13282. doi: 10.1039/c8cc06071a.

  10. Huang Y, Long Y, Li S, Lin T, Wu J, Zhang Y, Lin Y. Investigation on the processing and improving the cleavage efficiency of furin cleavage sites in Pichia pastoris. Microb Cell Fact. 2018 Nov 8;17(1):172. doi: 10.1186/s12934-018-1020-x.

  11.  Kuizhi Chen, Di Zeng, Ying Huang, Yawen Chen, Yide Huang, Shusen Xie, Yiru Peng & Qing Ye. Photophysical properties of pyridyloxy phthalocyanine encapsulated in nanoparticles. Spectroscopy letters, 2018 Jun; DOI: 10.1080/00387010.2018.1461654

  12. Huang Y, Lin M, Chen X, Huang C, Zhang X, Chen L, Wu K, Chen Y, Zhu Y, Lin Y.Evaluation of the prognostic and physiological functions of death associated protein kinase 1 in breast cancer. Oncol Lett. 2018 Jun;15(6):8261-8268.

  13. Huang Y, Zhang Y, Ge L, Lin Y, Kwok HF. The Roles of Protein Tyrosine Phosphatases in Hepatocellular Carcinoma. Cancers (Basel). 2018 Mar 20;10(3). pii: E82. doi: 10.3390/cancers10030082.  

  14.  Jiang N, You M, Wang Y, Huang Y, Xie S, Lin Y, Yang H. Probing the biophysical properties of tumor cells during mitosis by atomic force microscopy. biomech Model Mechanobiol. 2018 Mar 17. doi: 10.1007/s10237-018-1012-0.

  15.  Xiao Y, Meng C, Lin J, Huang C, Zhang X, Long Y, Huang Y, Lin Y. Ouabain targets the Na+/K+-ATPase α3 isoform to inhibit cancer cell proliferation and induce apoptosis. Oncol Lett. 2017 Dec;14(6):6678-6684. doi: 10.3892/ol.2017.7070. Epub 2017 Sep 26

  16.  Zhu Y, Li S, Wang Q, Chen L, Wu K, Huang Y, Chen X, Lin Y. Quantitative and correlation analysis of the DNA methylation and expression of DAPK in breast cancer. Peer J, 2017  5: 1-10 (doi:  10.7717/peerj.3084)

  17.  Xiao Y, Yan W, Guo L, Meng C, Li B, Neves H, Chen PC, Li L, Huang Y, Kwok HF, Lin Y. Digitoxin synergizes with sorafenib to inhibit hepatocelluar carcinoma cell growth without inhibiting cell migration. Mol Med Rep. 2017 15(2):941-947

  18.  Yu X, Lin T, Lv H, Pan S, Wu S, Zeng D, Jiang Y, Wang Y, Yang H, Huang Y*, Peng Y*. Photophysical properties of catechol axially substituted tetra-α-(pentyloxy) titanium(IV) phthalocyanine. Proc. of SPIE. 2016 10024: 100243P-1-100243P-5.   

  19. Huang Y, Yu H, Lv H, Zhang H, Ma D, Yang H, Xie S, Peng Y. Triblock copolymers encapsulated poly (aryl benzyl ether) dendrimer zinc(II) phthalocyanine nanoparticles for enhancement in vitro photodynamic efficacy. Photodiagnosis Photodyn Ther. 2016;16:124-131

  20. Huang Y, Zhang SD, McCrudden C, Chan KW, Lin Y, Kwok HF. The prognostic significance of PD-L1 in bladder cancer. Oncol Rep. 2015 33(6):3075-84. 

  21. Huang Y, Yang Y, Jiang M, Lin M, Li S, Lin Y Immortalization and characterization of human dental mesenchymal cells. J Dent. 2015 43(5):576-82.  

  22. Huang Y, Ma D, Pan S, Lin P, Lin Y, Yang H, Peng Y. Comparative study of aluminum phthalocyanine incorporating into two types of block copolymer: photophysical property, size, and in vitro photodynamic therapy efficacy. J Nanopart Res. 2015 17:41-50.

  23. Huang Y, Zhen R, Jiang M, Yang J, Yang Y, Huang Z, Lin Y. Development of all-in-one multicistronic Tet-On lentiviral vectors for inducible co-expression of two transgenes. Biotechnol Appl Biochem.  2015；62(1):48-54.

  24. Huang Y, Chen L, Guo L, Hupp T, Lin Y. Evaluating DAPK as a therapeutic target. Apoptosis (2014) 19:371–386

  25. Huang Y, Zhen B, Lin Y, Cai Y, Lin Z, Deng C, Zhang Y Expression of Codon Optimized Human Bone Morphogenetic Protein 4 in Pichia pastoris Biotechnol Appl Biochem.2014，61（2）：175-183.